二、FILL IN: （20 points, 1point for each) 1. Any pi...

二、FILL IN: (20 points, 1point for each blank) 1. The first few nucleotides at 5’-ends of Okazaki fragments and leading strand are ( ). 2. DNA ( ) travel along the template strands to open the double helix for copying. Binding of ( ) protein further promotes unwinding. 3. ( ) contains a short RNA molecule and acts as templet for the addition of these repeats to the 3’-overhang of DNA molecule by repeated cycles of elongation and translocation. 4. ( ) are widely used to repair all lesions of pyrimidine dimers. 5. In eukaryotes, the longer gaps generated in NER are filled by DNA polymerase ( ) or ( ). 6. The ( ) sequence appears to be the sequence at which DNA unwinding is initiated by the polymerase of prokaryotes. ( ) sequence offers the recognition signal of RNA holoenzyme. 7. Some known terminator sites may not form strong hairpins, and they use an accessory factor, the ( ) protein to mediate transcription termination. The protein hydrolyzes ( ) in the presence of ssRNA and actually it is a DNA-RNA ( ). 8. There are no tRNA species that normally recognize stop codons. ( )interact with these codons and cause release of the completed polypeptide chain. 9. ( ) is present in all three eukaryotic transcription complexes and clearly plays a major role in transcription initiation. It binds to ( ) box of promoter sequence. 10. Vectors must normally be capable of being ( ) and isolated independently of the host’s genome. Vectors also incorporate a ( ) marker. 11. ( ) libraries are prepared from random fragements of genomic DNA. 12. There are five important elements involved in PCR: ( ), Taq DNA polymerase, template, dNTP and ( ).

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III. Blank filling: Fill in each blank with a suitable word. tolerate preferable critical confront obsessed with benefit apt to deny carefree take up with 1. He was ________ a craving for materialistic gratification. 2. My father is not one to readily ________ new ideas. 3. I hope what I have written will be of ________ to someone else who may feel the same way. 4. The speaker hammered his point home with examples that the listeners could not ________. 5. He turned his face to look up at the sky in a ________ manner. 6. Environmentalists say a ________ factor in the city’s pollution is its population. 7. I was ________ fidget a good deal during the long performance. 8. Many people find gradual change ________ to sudden, large-scale changes. 9. They happily ________ the existence of opinions contrary to their own. 10. He ________ me with the task of designing and building the new system.

听力原文： A key point in the discussion of illegal immigration (and legal immigration, for that matter) is that many of those who are vehemently opposed to illegal immigration are also opposed to large amounts of legal immigration as well. These thinly hidden agendas mean that often the debate on illegal immigration cannot be separated from the debate on legal immigration.

Americans firmly believe' in tough laws against illegal immigrants and 70% of Americans want no more than 300,000 legal immigrants to enter the U. S. per year. In fact, 20% of Americans want immigration completely stopped. Taking these numbers as the truth, it is clear that America thinks that we have too many immigrants.

Such a dislike of immigration is interesting considering the success of past immigration. Many people would say that today's immigrants are somehow different than those of the past. However, the truth is that the similarities between the immigrants of today and those of the past are numerous. Their reasons for coming to this country are often similar.

When illegal immigration is not confused with legal immigration, debate can take place in a sane manner. Outlandish solutions like building a huge 2,000 mile-long fence and gathering a virtual army to defend the border from Mexicans are not the answer. Making legal immigration easier and more common is the best solution that we have now.

What is the most difficult part in the issue of illegal immigration, according to the passage you have just heard?

A．Legalizing all immigration, whether it is legal or illegal.

B．Dealing with the legal and illegal immigrations respectively.

C．Building a long fence along the long border near Mexico.

D．Making the legal immigration easier and more common.

Illegal Immigration

A key point in the discussion of illegal immigration (and legal immigration, for that matter) is that many of those who are vehemently opposed to illegal immigration are also opposed to large amounts of legal immigration as well. These thinly hidden agendas mean that often the debate on illegal immigration cannot be separated from the debate on legal immigration.

Americans firmly believe in tough laws against illegal immigrants and 70% of Americans want no more than 300,000 legal immigrants to enter the U.S. per year. In fact, 20% of Americans want immigration completely stopped. Taking these numbers as the truth, it is clear that America thinks that we have too many immigrants.

Such a dislike of immigration is interesting considering the success of past immigration. Many people would say that today's immigrants are somehow different than those of the past. However, the truth is that the similarities between the immigrants of today and those of the past are numerous. Their reasons for coming to this country are often similar.

When illegal immigration is not confused with legal immigration, debate can take place in a sane manner. Outlandish solutions like building a huge 2,000 mile-long fence and gathering a virtual army to defend the border from Mexicans are not the answer. Making legal immigration easier and more common is the best solution that we have now.

Which of the following solutions is the one the author will agree to?

A．Legalizing the illegal immigration.

B．Facilitating the legal immigration.

C．Building a long fence along the border.

D．Stationing a huge army defending the border.